Allelopathic Effect of Water Extracts from Rhizosphere Soil of Three Medicinal Plants on Polygala tenuifolia Seeds and Their Seedlings
10.13422/j.cnki.syfjx.20202076
- VernacularTitle:3种药用植物根际土壤水浸液对远志种子及其幼苗的化感作用
- Author:
Rui-xiang LEI
1
;
Bing-yue YANG
1
;
Yan-ni WEI
1
;
Fu-lin CAO
1
;
Yi-cong FAN
1
;
Sha-sha WU
1
;
Gang ZHANG
1
;
Yong-gang YAN
1
;
Liang PENG
1
;
Ben-xiang HU
1
Author Information
1. Qinling Chinese Herbal Medicine Application Development Engineering Technology Research Center，College of Pharmacy，Shaanxi University of Traditional Chinese Medicine，Xianyang 712046，China
- Publication Type:Research Article
- Keywords:
Polygala tenuifolia;
seed germination;
rhizosphere soil water extract;
allelopathic effect
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(23):161-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the allelopathic effects of water extracts from rhizosphere soil of three medicinal plants Rehmannia glutinosa，Pinellia ternata and Isatis indigotica on seed germination and seedling growth of Polygala tenuifolia， screen the stubble varieties suitable for crop rotation with P. tenuifolia， and provide some scientific basis for continuous cropping obstacles of P. tenuifolia. Method:The bioassay method was used to study the effects of rhizosphere soil water extracts from three medicinal plants Rehmannia glutinosa，Pinellia ternata and Isatis indigotica at concentrations of 0.3，0.6，0.9 g·mL-1 on the germination of P. tenuifolia seed and seedling growth. Result:The rhizosphere soil water extracts of Rehmannia glutinosa and Pinellia ternata showed basically low-promotion and high-inhibition concentration effects on the final germination rate，germination potential，and germination index of P. tenuifolia seeds，while the water extract of Isatis indigotica showed significant allelopathic inhibition effect. All three rhizosphere soil water extracts showed significant allelopathic inhibition effects on the growth index of P. tenuifolia seedlings. Among them，the rhizosphere soil water extract of Rehmannia glutinosa showed lower inhibitory effect on the plant height and root length of P. tenuifolia seedlings than the other two water extracts. The photosynthetic pigment content，proline（Pro） content，and soluble sugar content of P. tenuifolia chinensis seedlings were the highest under 0.3 g·mL-1 soil water extract of Rehmannia glutinosa， with relatively higher content of soluble protein， and relatively lower content of hydrogen oxide（H2O2）. Under the treatment of 0.9 g·mL-1 soil water extract of Rehmannia glutinosa，P. tenuifolia seedlings had the highest peroxidase（POD） and superoxide dismutase（SOD） activities，low catalase（CAT） activity，and lowest content of malondialdehyde（MDA）. Conclusion:Based on the comprehensive analysis of the above experimental data and allelopathic effects，the water extract of rhizosphere of Rehmannia glutinosa can promote the germination of P. tenuifolia seeds to a certain extent，and lay the foundation for seedling resistance to biochemical stress. Therefore， Rehmannia glutinosa is more suitable for crop rotation with P. tenuifolia.
