Genome-wide Identification and Characterization of TIFY Gene Family in Medicinal Plant Cannabis sativa
10.13422/j.cnki.syfjx.20202311
- VernacularTitle:中药火麻仁基原植物大麻的TIFY基因家族鉴定及功能分析
- Author:
Dong WEN
1
;
Meng-yue WANG
1
;
Yao-lei MI
1
;
Wei MA
2
;
Wei SUN
1
;
Yu-hua SHI
1
Author Information
1. Key Laboratory of Beijing for Identification and Safe Evaluation of Chinese Medicine，Institute of Chinese Meteria Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
2. College of Pharmaceutical Sciences，Heilongjiang University of Chinese Medicine， Harbin 150040，China
- Publication Type:Research Article
- Keywords:
bioinformatics;
Cannabis sativa;
TIFY gene family;
indetifine of members;
functional analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(24):134-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The TIFY gene family will be identified and characterized from the whole genome level in Cannabis sativa，which will lay the foundation for gene function study on TIFY family genes and their regulation mechanism on the biosynthesis of cannabinoids and other secondary metabolites. Method:Using the existing genomic data of cannabis，the CsTIFY genes were identified through bioinformatics analysis tools such as NCBI，PlantTFDB，MEME and TBtools etc.，and physicochemical properties，phylogenetic trees，gene structures，chromosome locations and gene expression patterns were analyzed and visualized. Result:Fourteen TIFY family genes（CsTIFY1-CsTIFY14） were identified in Cannabis sativa，which belong to four subfamilies：TIFY，JAZ，ZML，and PPD. The CsTIFYs are composed of 365-1 369 bp nucleotides encoding 118-442 amino acid residues，and their isoelectric points are 4.64-9.96. The 14 CsTIFYs are unevenly distributed on 8 chromosomes，and their proteins are all located in the nucleus. The promoter of CsTIFYs contain multiple abiotic stress responsive cis-acting elements，which indicated that CsTIFYs might involved in the regulation of different abiotic stresses. Transcriptome profiling revealed that CsTIFYs expressed differently in female flowers of 10 differently cannabis varieties，or in flowers，bracts，stems，and leaves of the same variety. Conclusion:Fourteen TIFY family genes were characterized from the whole genome level in C. sativa，and their phylogenetic evolutions and gene expression patterns were analyzed，indicating that CsTIFYs may play important regulatory roles in JA signal transduction，abiotic stress and cannabinoid biosynthesis. This study will provide valuable reference for gene function study of the TIFY family genes in cannabis and cannabis breeding.
