Protective Effect and Mechanism of Velvet Antler Peptide on Rotenone-induced SH-SY5Y Cell Damage
10.13422/j.cnki.syfjx.20201974
- VernacularTitle:鹿茸多肽对鱼藤酮致SH-SY5Y细胞损伤的保护作用及其机制
- Author:
Xu-fan ZHANG
1
;
Jun-ling CAO
2
;
Jin-tao HU
1
;
Wei LIU
2
;
Tian-quan WANG
3
;
Jing-feng OUYANG
4
Author Information
1. Dongzhimen Hospital， Beijing University of Chinese Medicine， Beijing 100700，China
2. Dongfang Hospital， Beijing University of Chinese Medicine， Beijing 100078，China
3. School of Traditional Chinese Medicine， Beijing University of Chinese Medicine， Beijing 102488，China
4. Medical Experimental Center， Chinese Academy of Chinese Medicine Sciences， Beijing 100700，China
- Publication Type:Research Article
- Keywords:
Parkinson's disease;
rotenone;
velvet antler polypeptide;
protein kinase B（Akt）;
mammalian target of rapamycin （mTOR）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(24):82-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the intervening effect of velvet antler peptide （VAP） on rotenone-induced neuroblastoma （SH-SY5Y） cell damage and explore its related mechanism. Method:0.5 μmol·L-1 rotenone was used to SH-SY5Y cells to establish an in vitro model of Parkinson's disease （PD）. A blank control group， a model group， high， medium and low dose VAP groups （150，100，50 mg·L-1， respectively） and a rapamycin group were established. The number of lewy bodies， changes in mitochondrial membrane potential， content of reactive oxygen species （ROS） and α-synuclein （α-syn）， protein kinase B （Akt）， and mammalian target of rapamycin （mTOR） were observed by hematoxylin-eosin（HE） staining， rhodamine 123 staining， DCFH-DA staining and immunohistochemical staining expression respectively. Result:The results of HE staining showed that as compared with the blank group， the number of cells in model group was reduced， the tentacle structure became dull， the shape became round， and eosinophilic Lewy bodies were visible in cytoplasm. As compared with model group， there was no significant difference in cell morphology from rapamycin group and VAP high， medium and low dose groups， but there were fewer Lewy bodies in cytoplasm in these four groups. Rhodamine 123 staining showed that as compared with blank group， the mitochondrial membrane potential was increased significantly in model group （P<0.05）. As compared with the model group， the mitochondrial membrane potential was decreased in rapamycin group and VAP high， medium and low dose groups （P<0.05）. DCFH-DA staining results showed that as compared with blank group， the content of ROS was increased significantly in cells of model group （P<0.05）. As compared with model group， the content of ROS was decreased in rapamycin group and VAP high， medium and low dose groups （P<0.05）. Immunohistochemical staining showed that as compared with blank group， the protein expression levels of α-syn，Akt，and mTOR were increased significantly in model group （P<0.05）. As compared with model group， the protein expression levels of α-syn and mTOR were significantly reduced in rapamycin group and VAP high and medium dose groups （P<0.05）， and the expression levels of Akt were significantly reduced in rapamycin group and VAP high-dose group （P<0.05）. Conclusion:Velvet antler peptides may play a neuroprotective role by regulating the Akt/mTOR signaling pathway and promoting the degradation of α-syn in SH-SY5Y cells.
