Licochalcone A Inhibits Proliferation and Induces Apoptosis of MH7A Cells by Regulating MAPK Signaling Pathway
10.13422/j.cnki.syfjx.20202439
- VernacularTitle:甘草查尔酮A通过调控MAPK信号通路抑制MH7A细胞增殖并诱导凋亡
- Author:
Wan-yi GUO
1
;
Bei YUAN
2
;
Qian WANG
2
;
Xiao-hui SU
2
Author Information
1. School of Pharmaceutical Sciences，Guangzhou University of Chinese Medicine，Guangzhou 510006，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medicine Sciences，Beijing 100700，China
- Publication Type:Research Article
- Keywords:
licochalcone A;
fibroblast-like synoviocytes（MH7A）cells;
mitogen activated protein kinase （MAPK） signaling pathway;
apoptosis;
interleukin-1β（IL-1β）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2020;26(24):68-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of licochalcone A （LCA） on the proliferation and apoptosis of rheumatoid arthritis fibroblast-like synoviocytes （MH7A） as well as the related inflammatory factors， also to reveal the relevance between mitogen activated protein kinase （MAPK） signaling pathway and LCA regulation of MH7A cell proliferation and apoptosis. Method:MH7A cells were cultured and divided into blank group， LCA groups （10，20，40 μmol·L-1）. The proliferation of MH7A cells was detected by methylthiazolyldiphenyl-tetrazolium bromide（MTT）and immunofluorescence staining. The cell cycle of MH7A cells was determined by flow cytometry after PI staining and apoptosis was detected by flow cytometry after Annexin V/PI staining. The effect of LCA on interleukin-1β（IL-1β） mRNA was detected by Real-time fluorescence quantitative polymerase chain reaction（Real-time PCR）. Western blot was used to detect the effect of LCA on the key proteins of MAPK signaling pathway， meanwhile， PD98059， a specific ERK inhibitor， was used to observe the expression levels of p-ERK and IL-1β. Result:Compared with blank group， LCA could inhibit the proliferation of MH7A cells in a dose-dependent manner， and the number of living cells decreased significantly（P<0.01）， while the number of early apoptotic cells increased significantly（P<0.01）. Compared with the tumor necrosis factor-α （TNF-α，10 μg·L-1）group， LCA could reverse the expression of IL-1β mRNA induced by TNF-α（P<0.01）. and compared with the blank group， LCA also promoted the phosphorylation of ERK， JNK and p38 in a dose-dependent manner（P<0.01）. After ERK inhibitor PD98059 inhibited ERK phosphorylation， the inhibitory effect of LCA 10， 20 μmol·L-1 on IL-1β disappeared. Conclusion:LCA can inhibit the proliferation and induce apoptosis of MH7A cells， which may be related to the phosphorylation of MAPK pathway related proteins， and then inhibit the expression of inflammatory factors.
