Expression of miR-126 in oral squamous cell carcinoma tissues and its clinical significance
10.3872/j.issn.1007-385X.2021.01.008
- VernacularTitle:miR-126在口腔鳞状细胞癌组织中的表达及其临床意义
- Author:
GAO Yongqianga
1
;
SHI Pengweib
2
;
LIU Xiaoxiaoa
1
;
SHI Wenkaic
3
;
LIU Yimingb
2
Author Information
1. a. Department of Oral Periodontal
2. b. Department of Oral and Maxillofacial Surgery
3. c. Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan, China
- Publication Type:Journal Article
- Keywords:
miR-126;
oral squamous cell carcinoma (OSCC);
Tca8113 cell;
proliferation;
apoptosis;
invasion;
prognosis
- From:
Chinese Journal of Cancer Biotherapy
2021;28(1):48-54
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective: To detect the expression of miR-126 in oral squamous cell carcinoma (OSCC) and to analyze its correlation with clinicopathological features and prognosis of patients, as well as to explore the effect of miR-126 over-expression on the malignant biological behaviors of Tca8113 cells. Methods: A total of 62 pairs of cancer and para-cancerous tissue specimens from OSCC patients who were surgically treated in the First Affiliated Hospital of Zhengzhou University from June 2016 to June 2018 were collected for this study; in addition, human tongue squamous carcinoma Tca8113 cell line and human mouth keratinocyte HOK cell line were also selected for this study. The expression of miR-126 in cancer tissues and cells was detected by qPCR, and the relationship between miR-126 expression and clinicopathological features and prognosis of the patients was analyzed. miR-126 mimics and miR-NC plasmids were respectively transfected into Tca8113 cells by liposome transfection technology. Cell proliferation, apoptosis, migration and invasion were detected by MTT method, Flow cytometry and Transwell chamber method, respectively; and the expressions of apoptosis, migration and invasion related proteins were detected by Western blotting. Results: The expression level of miR-126 in OSCC tissues and Tca8113 cells was significantly lower than that in para-cancerous tissues and HOK cells (all P<0.01). The expression of miR-126 was associated with TNM stage and lymph node metastasis (all P<0.05), and patients with high miR-126 expression had significantly better overall survival rate than patients with low expression (P<0.05). After transfection with miR-126 mimics, the cell proliferation, migration and invasion ability significantly decreased (P<0.05 or P<0.01) while the apoptosis rate significantly increased in Tca8113 cells (P<0.01), the expression levels of Bcl-2, N-cadherin and vimentin in Tca8113 cells significantly decreased (all P<0.01), and expression levels of Bax and E-cadherin significantly increased (all P<0.01). Conclusion: miR-126 is low expressed in OSCC tissues and Tca8113 cells. Up-regulation of miR-126 inhibits cell proliferation, migration and invasion and promotes apoptosis of Tca8113 cells.
- Full text:20210108.pdf
