Comparative study on the effects of total ginkgo flavonol glycosides and total ginkgolides on neurofunctional recovery in mice with subacute stroke
10.16438/j.0513-4870.2020-1265
- VernacularTitle:银杏总黄酮与银杏总内酯对亚急性期卒中小鼠神经功能恢复促进作用的比较研究
- Author:
Zhi-xiong LI
1
,
2
;
Guang-xu XIAO
1
,
2
;
Shuang HE
1
,
2
;
Huan-yi WANG
1
,
2
;
Yan ZHU
1
,
2
Author Information
1. State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
2. Chinese Medicine New Drug Research and Development Center, Tianjin International Biomedical Research Institute, Tianjin 300457, China
- Publication Type:Research Article
- Keywords:
shuxuening injection;
total ginkgo flavonol glycosides;
total ginkgolides;
ischemic stroke;
granulocyte colony-stimulating factor
- From:
Acta Pharmaceutica Sinica
2021;56(1):231-243
- CountryChina
- Language:Chinese
-
Abstract:
As a Ginkgo biloba extract preparation, shuxuening injection has a unique advantage in the prevention and treatment of acute and subacute stroke, but its main active ingredient is still unclear. Using a subacute model of stroke in mice constructed earlier, we further explored the contribution and mechanism of the two main components of total ginkgo flavonol glycosides and total ginkgolides in facilitating the neurofunctional recovery in stroke-induced mice. The pharmacodynamics was mainly evaluated by neurobehavioral changes, cerebral infarction volume, blood-brain barrier permeability and brain edema. The pathway and targets were predicted by transcriptome and network pharmacology. Finally, the mechanism was verified at the mRNA and protein levels. The results showed that the beneficial effect of total ginkgolides was greater than that of total ginkgo flavonol glycosides in both the pharmacodynamics and the regulatory mechanism of granulocyte adhesion and diapedesis involving granulocyte colony-stimulating factor (G-CSF), macrophage-1 antigen (MAC-1) and E-selectin. These findings suggest that shuxuening injection may improve the prognosis for mice with subacute stroke by down-regulating G-CSF-mediated granulocyte adhesion and diapedesis pathway mainly through the total ginkgolide components. This finding is expected to provide reference for optimizing prescription and searching for natural drugs for targeting the treatment of ischemic stroke prognosis. The animal experiments in this study followed the regulations of Animal Ethics Committee of Tianjin University of Traditional Chinese Medicine.
