Correlations of serum miRNA-21 level with clinicopathological factors and prognosis of patients with cervical cancer
10.3760/cma.j.cn115355-20190830-00391
- VernacularTitle:子宫颈癌患者血清miRNA-21水平与临床病理因素及预后的关系
- Author:
Dan LIU
1
;
Minghui CAI
;
Li WEI
Author Information
1. 中国医科大学附属第四医院妇科，沈阳 110032
- From:
Cancer Research and Clinic
2020;32(5):313-317
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the relationship between the level of serum miRNA-21 (miR-21) and clinicopathological factors and prognosis of patients with cervical cancer.Methods:Seventy-two patients with cervical cancer diagnosed by pathological examination in the Fourth Affiliated Hospital of China Medical University from August 2013 to January 2016 were selected (cervical cancer group), and 60 cases without cervical lesions through cervical examination were taken as the healthy control group. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the level of serum miR-21 in both groups. The differences of serum miR-21 levels in cervical cancer patients with different pathological characteristics were analyzed. Cox proportional hazards regression model was used to analyze the factors affecting the survival of cervical cancer patients.Results:The preoperative relative expression of serum miR-21 in the cervical cancer group was significantly higher than that in the healthy control group (9.64±2.01 vs. 6.38±1.42, t = 10.556, P < 0.05). The preoperative relative expression of serum miR-21 in patients with the International Federation of Gynecology and Obstetrics (FIGO) stage Ⅲ-Ⅳ (38 cases) was higher than that in patients with stage Ⅰ-Ⅱ (34 cases) (10.14±1.96 vs. 9.10±1.88), the difference was statistically significant ( t = -2.291, P = 0.025). The preoperative relative expression of serum miR-21 in patients with lymph node metastasis (36 cases) was higher than that in patients without lymph node metastasis (36 cases) (10.32±1.80 vs. 8.91±1.94), the difference was statistically significant ( t = 3.197, P = 0.002). The preoperative relative expression of serum miR-21 in surviving patients (29 cases) was lower than that in death patients (43 cases) (9.23±1.93 vs. 10.47±1.89), the difference was statistically significant ( t = 2.696, P = 0.009). There was no statistical difference in relative expression of serum miR-21 among cervical cancer patients with different ages, pathological types, degree of differentiation, and depth of tumor infiltration (all P > 0.05). According to the Cox proportional hazards regression model analysis, the late FIGO stage, lymph node metastasis and high preoperative relative expression of serum miR-21 were independent risk factors for the prognosis of cervical cancer patients ( OR = 3.789, 95% CI 1.106-12.973, P = 0.048; OR = 3.228, 95% CI 1.116-9.34, P = 0.046; OR = 1.714, 95% CI 1.079-2.723, P = 0.039). Conclusion:The high relative expression of serum miR-21 in patients with cervical cancer is related to the high degree of tumor malignancy and the poor prognosis.
