Survival mechanism of free bone graft in calvarial defects using a murine model
10.3760/cma.j.issn.1671-0290.2020.04.003
- VernacularTitle:游离骨移植在小鼠颅骨缺损中的成活机制研究
- Author:
Rui HOU
1
;
Shanshan BAI
;
Bokai FAN
;
Liang XU
;
Zheyuan YU
;
Min WEI
Author Information
1. 上海交通大学附属第九人民医院整复外科 200011
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2020;26(4):268-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of free bone graft survival in calvarial defects.Methods:We simulated the bone autograft reconstruction through transplanting green fluorescent protein (GFP) mice＇ cranium to the nude mice＇ calvarial bone defects. Then gross and histological evaluation were performed to calculate BTA (%) and GFP (%) in graft bone.Results:The results showed that the green fluorescence was getting disappeared from 4 to 24 weeks＇ post-operation. However, a small amount of green fluorescence remained at 24 weeks＇ post-operation (6.69% of the positive control group). In addition, the gross and histological evaluation indicated that the grafted bone survived well at different time points.Conclusions:The survival mechanism of free bone graft in cranium mainly relies on creeping substitution assisted with bony regeneration.
