Establishment of a candidate reference method for serum glycated albumin based on liquid chromatography tandem mass spectrometry
10.3760/cma.j.cn114452-20200527-00504
- VernacularTitle:同位素稀释液相色谱串联质谱测定血清糖化白蛋白
- Author:
Huijuan ZHOU
1
;
Tianjiao ZHANG
;
Qichen LONG
;
Jie ZENG
;
Jiangtao ZHANG
;
Weiyan ZHOU
;
Chao ZHANG
;
Chuanbao ZHANG
Author Information
1. 北京医院 国家老年医学中心 国家卫生健康委临床检验中心 北京市临床检验工程技术研究中心 中国医学科学院老年医学研究院 中国医学科学院北京协和医学院，北京100730
- From:
Chinese Journal of Laboratory Medicine
2020;43(10):984-989
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an accurate quantitative method for the determination of serum glycated albumin (GA) based on isotope dilution liquid chromatography tandem mass spectrometry (ID-LC/MS/MS).Methods:This study optimized the ID-LC/MS method recommended by the Japan Society of Clinical Chemistry (JSCC). Albumin (Alb) was purified by anion exchange chromatography. The standard solution of purified sample, lysine (Lys) and deoxyfructosyl lysine (DOF-Lys) and their mixed internal standard were weighed accurately by weight method. The Alb was hydrolyzed to amino acid, and Lys and DOF-Lys were detected to determine GA. The method was evaluated and compared with the routine method.Results:The purity of Alb was 100%. The R 2 ranges of Lys and DOF-Lys linear standard curves were 0.997 8-0.999 9 and 0.999 4-1.000 0. The results of three-day precision evaluation of low, medium and high concentrations of serum were 0.69%-1.97%, 1.13%-2.33% and 1.37%-2.54% in intra, inter and total Coefficient of variation ( CV). In the accuracy evaluation, the deviations between the results of three concentration standards and the certified value were -2.56%, -1.87% and-2.94%. The matrix effects of Lys and DOF-Lys were 89.92% and 109.98%, and the carryover rates were -1.13% and -3.27% respectively. The detection limit and quantitative limit of Lys were 0.075 nmol/g and 0.248 nmol/g, and the DOF-Lys were 0.007 nmol/g and 0.024 nmol/g. The relative expanded uncertainty was 1.60%-2.03%. The fitting regression curve R 2 with the routine method was 0.970 7. Conclusions:A method was established for the detection of serum GA. The method evaluation was accurate and reliable. It was expected to be a candidate reference method for the detection of serum GA.
