Principle and application of isothermal amplification technology
10.3760/cma.j.cn114452-20190903-00510
- VernacularTitle:等温扩增技术的原理及应用
- Author:
Su JIANG
1
;
Yirong LI
Author Information
1. 武汉大学中南医院检验科，武汉 430071
- From:
Chinese Journal of Laboratory Medicine
2020;43(5):591-596
- CountryChina
- Language:Chinese
-
Abstract:
Isothermal amplification technology is a new class of nucleic acid amplification technology that performs amplification under constant temperature conditions. With the advantages of simple operation, high sensitivity and specificity, this kind of technology shows a good application prospect in clinical detection and scientific research. The principle, characteristics and applications of loop-mediated isothermal amplification(LAMP), crossing priming amplification (CPA),strand displacement amplification(SDA), recombinase polymerase amplification (RPA), nucleic acid sequence-based amplification (NASBA), rolling circle amplification (RCA) and helicase-dependent amplification (HDA) are reviewed in this paper.
