Applications and challenges of metagenomic next-generation sequencing in the detection of 2019 novel coronavirus
10.3760/cma.j.issn.1009-9158.2020.03.004
- VernacularTitle:病原宏基因组测序在新型冠状病毒检测中的应用与挑战
- Author:
Yue TAO
1
;
Qihua FU
;
Xi MO
Author Information
1. 国家儿童医学中心 上海交通大学医学院附属上海儿童医学中心精准医疗实验诊断中心，上海 200127
- From:
Chinese Journal of Laboratory Medicine
2020;43(3):217-220
- CountryChina
- Language:Chinese
-
Abstract:
As one of the two methods for 2019 novel coronavirus (2019-nCoV), gene sequencing is different from quantitative real-time PCR (RT-PCR) in detection principles. Therefore, gene sequencing has its own pros and cons in clinical application. Currently, metagenomic next-generation sequencing (mNGS) is the most commonly used technology in clinical application.Due to its broad coverage of all types of pathogens, mNGS demonstrates incomparable advantage in rapid identification of novel pathogens such as 2019-nCoV. In addition, it can simultaneously identify other pathogens except 2019-nCoV and mixed infections. On the other hand, however, due to the complexity of mNGS and long detection time, it is unlikely to achieve the purpose of wide-range and rapid diagnosis of 2019 n-CoV. Therefore, mNGS can complement RT-PCR to achieve best clinical application.
