Evaluation of interference factors in creatinine measurement by using new guideline CLSI EP7-A3
10.3760/cma.j.issn.1009-9158.2020.03.021
- VernacularTitle:应用CLSI EP7-A3评价肌酐测定的干扰因素
- Author:
Wen SHI
1
;
Jieru HUANG
;
Dongdong LIU
;
Min HE
;
Chuhong ZHENG
;
Qian XIAO
;
Li LIN
;
Yile HUANG
;
Lin LI
;
Jianhua XU
Author Information
1. 广东省中医院检验医学部，广州 510105
- From:
Chinese Journal of Laboratory Medicine
2020;43(3):307-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the interfering factors in the determination of creatinine(Cr) using the American Clinical Laboratory Standards Association (CLSI) EP7-A3 document.Methods:According to the CLSI EP7-A3 document, fresh serum (no hemolysis, lipemia, and jaundice) was used on the day of the experiment and confirmed the interfering substances through the pairing difference experiment and the point-to-point analysis method was used in the dose effect experiment to clarify the difference of interfering substances.Results:Triglyceride (16.94 mmol/L), dobutamine hydrochloride (4.01 μmol/L), ascorbic acid (298 μmol/L) did not interfere with the determination of Cr. Free bilirubin (684 μmol/L), conjugated bilirubin (684 μmol/L), calcium hydroxybenzene sulfonate (144 μmol/L) and hemoglobin (10 g/L) were used as the maximum concentrations of interferences for the dose effect test, the results showed that the above interferences had negative interference on the determination of Cr.Conclusion:According to EP7-A3, it is valuable to evaluate the interference factors of creatinine determination.
