Upregulation of IP-10(CXCL10) mRNA Expression by Interleukin-18.
10.12701/yujm.2007.24.1.67
- Author:
Hyo Young KIM
;
Hee Sun KIM
- Publication Type:Original Article
- Keywords:
IP-10(CXCL10);
Interleukin-18;
Lipopolysaccharide
- MeSH:
Animals;
Blotting, Northern;
Electrophoretic Mobility Shift Assay;
Interferon-gamma;
Interleukin-18*;
Lymphocytes;
Macrophages, Peritoneal;
Mice;
NF-kappa B;
RNA, Messenger*;
Up-Regulation*
- From:Yeungnam University Journal of Medicine
2007;24(1):67-78
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Interleukin-18 (IL-18) is one of the principal inducers of interferon-gamma (IFN-gamma) in lymphocytes. MATERIALS AND METHODS: The effect of IL-18 on the expression of chemokine IP-10(CXCL10) mRNA in C57BL/6 mouse peritoneal macrophages was studied by using Northern blot analysis, enzyme linked immunosobent assay and electrophoretic mobility shift assay. RESULTS: IL-18 was determined to exert no direct effect on the expression of IP-10(CXCL10) mRNA. However, IL-18 pretreatment was determined to play a cooperative role in the synergistic induction of LPS-induced IP-10(CXCL10) mRNA expression. The effect associated with IL-18 pretreatment with regard to the synergistic induction of LPS-induced IP-10 (CXCL10) mRNA expression was detected after 16 hr of IL-18 pretreatment, administered prior to LPS stimulation. The pattern of NF-kB binding activity during IL-18 pretreatment with LPS stimulation was found to coincide with the expression of IP-10(CXCL10) mRNA. CONCLUSION: Although IL-18 alone exerts no direct effect on the expression of chemokine IP-10(CXCL10), a definite period of IL-18 pretreatment induces the synergistic expression of LPS-induced IP-10(CXCL10) mRNA. NF-kB activation is a component of this synergistic effect of IL-18 pretreatment. These results provide useful information, which may facilitate the elucidation of the action mechanisms underlying IL-18 effect on the expression of IP-10(CXCL10) mRNA.
