Current situation of vitreous and retinal related tissue specimens collection and application
10.3760/cma.j.cn511434-20190312-00091
- VernacularTitle:玻璃体和视网膜相关组织标本取材及应用研究现状
- Author:
Qiong CHEN
1
;
Chaohui CHENG
;
Bojie HU
Author Information
1. 天津医科大学眼科医院、眼视光学院、眼科研究所 天津市视网膜功能与疾病重点实验室天津市眼科学与视觉科学国际联合研究中心 300384
- From:
Chinese Journal of Ocular Fundus Diseases
2020;36(5):396-399
- CountryChina
- Language:Chinese
-
Abstract:
Accurate collection and preservation of vitreous and retina-related tissue specimens is the basis for clinical diagnosis and rigorous basic research. The clinical uses of vitreous specimens include microbial culture, cytological detection, detection of degenerative diseases, PCR analysis, and cytological detection of cell morphology. The experimental research uses include DNA gene analysis, protein quantitative analysis, metabolite examination, RNA content quantitative analysis, cytokine determination and so on. Retinal specimens collecting was mainly used for PCR analysis of retinal proliferative membrane, immunohistochemical staining, immunofluorescence examination, microvascular density evaluation, cell isolation and culture, etc. Understanding the collection of vitreoretinal surgical specimens and the application of relevant detection techniques and materials can provide a more comprehensive idea for the diagnosis of vitreoretinal diseases and a broader reference for the related basic research.
