Study of gene correlation between endothelial growth factor family in mouse eye

Jinglin Cui; Hang LU; Jing Chen; Binjiang Luo; Weiwei Xiu; Huimei LI; Jiao Huang; Yuhan Chen; Jingyu Chen

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Study of gene correlation between endothelial growth factor family in mouse eye

VernacularTitle:小鼠眼血管内皮生长因子家族基因相关性研究
Author: Jinglin CUI ¹ ; Hang LU ; Jing CHEN ; Binjiang LUO ; Weiwei XIU ; Huimei LI ; Jiao HUANG ; Yuhan CHEN ; Jingyu CHEN
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1. 黑龙江省齐齐哈尔市第一医院眼科　161005
From: Chinese Journal of Ocular Fundus Diseases 2020;36(3):211-219
CountryChina
Language:Chinese
Abstract: Objective:To compare the function and action pathways of VEGFA, VEGFB and VEGFC in VEGF family of mouse eye.Methods:Using the BXD mouse gene data in Genenetwork database as template to compare and study the similarities and differences of VEGFA, VEGFB and VEGFC molecular pathways or potential functions in the whole genome expression spectrum of BXD recombinant mouse inbred line population, with multiple analytical methods and statistical strategies were used, such as gene expression level, target genes comparison, top genes comparison associated to target genes, expression Quantitative Trait Loci (eQTL).Results:Matrix comparison showed strong positive correlation between two probes of VEGFC ( r=0.732, P<0.01), weak correlation between VEGFA 1420909 and VEGFC 1440739, VEGFA 1451959 and VEGFC 1451803, VEGFC 1419417959 and VEGFC 1439766, VEGFC 1451803 and VEGFC 1439766 ( P<0.05); there was no correlation between VEGFA 1420909 and four other genes except VEGFC 1440739, VEGFA 1451959 and VEGFC 1440739, VEGFB 1451803 and VEGFA 1420909/VEGFC 1419417/VEGFC 1440739 ( P >0.05). In the comparative analysis of the relevant Top 50 genes of each VEGF gene, most of the genes in BXD mouse were not significantly correlated with VEGFA, VEGFB and VEGFC except for the weak association of individual related genes. The results of eQTL analysis showed that each probe of VEGF gene was located on different chromosomes. Conclusions:The expression levels and positive and negative correlations of VEGFA, VEGFB and VEGFC were different in the VEGF family of mouse eye, suggesting that these genes may play their role through different pathways.