Application of virus-mediated gene transduction technology in ophthalmology research
10.3760/cma.j.issn.1005-1015.2020.02.019
- VernacularTitle:以病毒为载体的基因转导技术在眼科研究中的应用
- Author:
Lijie DONG
1
;
Hui ZHANG
;
Qiong WANG
;
Yaru HONG
;
Xiaorong LI
Author Information
1. 天津医科大学眼科医院 天津医科大学眼科研究所 天津医科大学眼视光学院 300384
- From:
Chinese Journal of Ocular Fundus Diseases
2020;36(2):165-171
- CountryChina
- Language:Chinese
-
Abstract:
With the advancement of molecular biology technology and the development of genetics, the viral vector system has been continuously improved and optimized. The viral vector system has gradually become one of the best carriers in ophthalmic gene therapy. Adenovirus vector has the characteristics of transient expression and plays an important role in reducing corneal immune response. Lentiviral vector has the characteristics of stable and high efficiency and can be expressed slowly in the body for a long time.Adeno-associated virus vector has the characteristics of low immunogenicity, high efficiency and precision and can infect a variety of retinal cells. The combined use of adeno-associated virus vector and CRISPR-Cas9 provides new methods for precise treatment of ophthalmic genetic diseases. The advent of viral vectors has significantly increased the potential of gene therapy and has unparalleled advantages over traditional therapies. We have reason to believe that virus-based gene transduction technology will soon achieve clinical application in the near future, and a large number of difficult ophthalmic problems will be solved by then.
