Comparative analysis of conditions for culturing influenza virus H1N1 vaccine strain in MDCK and MDCK-G1 cell lines
10.3760/cma.j.cn112309-20200509-00251
- VernacularTitle:H1N1疫苗株在MDCK和MDCK-G1细胞中的培养条件比较
- Author:
Xinghang LI
1
;
Chen LIU
;
Jiayou ZHANG
;
Zhegang ZHANG
;
Xuanxuan NIAN
;
Zheng GONG
;
Ziyan MENG
;
Ran QIU
;
Qingmei ZHANG
;
Xiaoming YANG
Author Information
1. 武汉生物制品研究所有限责任公司病毒性疫苗研究二室 430207
- From:
Chinese Journal of Microbiology and Immunology
2020;40(11):870-875
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the optimal conditions, virus yield, viral titer and cell metabolism between culturing influenza virus H1N1 vaccine strain in MDCK and MDCK-G1 cells.Methods:The optimal culture conditions were investigated using chessboard method. The hemagglutination titer, half of the tissue infection dose (TCID 50) and the metabolism of glucose and lactic acid were monitored and compared between the two cell lines. Results:After MDCK-G1 cells were inoculated with H1N1 at the multiplicity of infection (MOI) of 0.001 with the presence of 1 μg/ml of trypsin, the hemagglutination titer reached the peak of 1∶512 at 72 h and the viral titer was 10 7.4TCID 50/ml. In the MDCK cell line group, the hemagglutination titer reached the peak of 1∶256 at 72 h and the viral titer was 10 6.6TCID 50/ml when using H1N1 at MOI=0.0001 and 1 μg/ml of trypsin. Conclusions:MDCK-G1 cells were more suitable than MDCK cells for the proliferation of influenza virus. This study provided reference data for further research on cell-derived influenza vaccine.
