Brucella suis vaccine strain S2 induces apoptosis of murine BV-2 microglia cells via JNK-p53 signaling pathway
10.3760/cma.j.cn112309-20200801-00383
- VernacularTitle:布鲁杆菌S2株通过JNK-p53信号通路诱导小鼠BV-2小胶质细胞凋亡
- Author:
Zhao WANG
1
;
Boya MA
;
Jiayu GUO
;
Ru HE
;
Yanbai WANG
;
Qiang LIU
;
Zhenhai WANG
Author Information
1. 宁夏医科大学临床医学院,银川 750004
- From:
Chinese Journal of Microbiology and Immunology
2020;40(11):857-862
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the potential mechanism by which Brucella suis vaccine strain S2 induces the apoptosis of BV-2 microglia cells, and to discover new protein targets for neurobrucellosis treatment. Methods:BV-2 microglia cells were treated with Brucella suis vaccine strain S2 for 0, 3, 6, 12 and 24 h. Western blot assay and RT-qPCR were performed to detect the expression of p-JNK and p53 at protein and mRNA levels in BV-2 microglia cells. Cell apoptosis was measured by flow cytometry. Immunofluorescence was used to analyze nuclear p-JNK. Results:Brucella suis vaccine strain S2 could promote the expression of p-JNK and p53 at both protein and mRNA levels and increase nuclear p-JNK in BV-2 microglia cells. Moreover, it could also induce the apoptosis of BV-2 microglia cells. Conclusions:Brucella suis vaccine strain S2 could promote the apoptosis of BV-2 microglia cells through activating JNK and promoting p53 expression.