In vitro inhibitory effects of emodin on Staphylococcus aureus biofilm formation
10.3760/cma.j.cn112309-20200225-00079
- VernacularTitle:大黄素对金黄色葡萄球菌生物膜抑制作用的体外研究
- Author:
Qi CHEN
1
;
Xiuqin LOU
;
Tao LIU
;
Wei ZHANG
Author Information
1. 杭州市疾病预防控制中心微生物检验科 310021
- From:
Chinese Journal of Microbiology and Immunology
2020;40(9):703-708
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of emodin on Staphylococcus aureus ( S. aureus) biofilm formation and disruption. Methods:ATCC6538 and S. aureus 16-22 were used in this study. The minimum inhibitory concentrations (MICs) of emodin against the two strains were detected by the standardized agar dilution method. After an in vitro biofilm model was established, silver staining method was used to observe the bacterial cell morphology; crystal violet staining and double dilution method were performed to measure the viable count of bacteria in biofilms. Results:The MICs of emodin against ATCC6538 and 16-22 were 32 μg/ml and 16 μg/ml, respectively. The bacterial cell morphology showed that ≥4 μg/ml emodin effectively reduced the adherence of S. aureus to the plate and destroyed mature biofilms. The results of crystal violet staining and double dilution method showed that when the concentration of emodin reached to 8 μg/ml, 75% of ATCC6538 and 70% of 16-22 failed to form biofilms and 56% of the mature biofilms on the plate were broken. Conclusions:Emodin could effectively inhibit the biofilm formation and destroy the mature biofilms of S. aureus in vitro.
