Neutralization properties of different genotypes and mutants of severe fever with thrombocytopenia syndrome virus
10.3760/cma.j.cn112309-20200116-00030
- VernacularTitle:发热伴血小板减少综合征病毒不同基因型和变异株的中和特性分析
- Author:
Ruifeng CHEN
1
;
Weijin HUANG
;
Jiajing WU
;
Mifang LIANG
;
Yanhua DU
;
Hongxia MA
;
Yuhua LI
;
Youchun WANG
Author Information
1. 国家药品监督管理局生物制品质量评价重点实验室，北京 102629；中国食品药品检定研究院生物制品检定所艾滋病性病病毒疫苗实验室，北京 102629
- From:
Chinese Journal of Microbiology and Immunology
2020;40(8):607-615
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the neutralization properties of different genotypes and mutants of severe fever with thrombocytopenia syndrome virus (SFTSV).Methods:Pseudoviruses of SFTSV of different genotypes and mutants were constructed using VSVΔG-Fluc*G backbone. Neutralization assays were established based on the pseudoviruses. DNA vaccines for different SFTSV genotypes were prepared. Serum samples were collected from guinea pigs immunized with the DNA vaccines. Neutralizing antibodies in serum samples from immunized guinea pigs and naturally infected patients were detected using neutralization assays and analyzed.Results:The pseudoviruses of five genotypes and 43 mutants were successfully constructed and the neutralization assays based the pseudoviruses were successfully established after optimizing the reaction parameters. The dilution multiple corresponding to the inhibition rate of neutralizing antibody to half of the pseudovirus infection was taken as the titer of neutralizing antibody by the reduction in pseudovirus reporter gene. The neutralization antibody titers in naturally infected patients and immunized guinea pigs were respectively in the ranges of 1∶100-1∶43 000 and 1∶100-1∶2 500 when detected with the reference HB29 pseudovirus. The neutralization antibody titers ranged from 1∶100-1∶2 500 after immunization with different genotypes of DNA vaccines. No significant statistical difference in neutralization antibody titer was observed among different genotypes or mutant strains.Conclusions:The neutralization properties of different genotypes and mutants showed no significant change, which would be very useful for developing vaccines.
