Binding epitopes of HIV-1 broad neutralization antibodies
10.3760/cma.j.cn112309-20191206-00412
- VernacularTitle:HIV-1广谱中和抗体的结合表位研究
- Author:
Dai ZHANG
1
;
Yuanyuan HU
;
Shasha SUN
;
Sen ZOU
;
Weihong REN
Author Information
1. 河南中医药大学第一附属医院检验科，郑州 450000；中国疾病预防控制中心性病艾滋病预防控制中心病毒免疫室，北京 102206
- From:
Chinese Journal of Microbiology and Immunology
2020;40(7):553-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the potential specificity of broad neutralizing antibodies (bNAbs) in one patient (DRVI01) with chronic HIV-1 infection.Methods:Sequences of the envelope glycoprotein (Env) obtained from DRVI01 at different time points were analyzed by comparing with the key amino acids of reported HIV-1 bNAbs in HIV Database. After reverse mutation of key amino acids that had frequently mutated to wild type, the neutralizing sensitivity of autologous plasma against wild-type and mutated Env-pesudoviruses was compared and the potential bNAbs in DRVI01 were speculated.Results:Reported key amino acids of 10 bNAbs classes were detected in 155 Env sequences derived from DRVI01. Frequent mutations were found in key amino acids of two bNAbs classes of gp41 fusion domain and gp120/gp41 interface. Neutralizing sensitivity of the contemporaneous autologous plasma and the plasma collected at the next time point against the mutated pesudoviruses was significantly increased as compared with wild-type pesudoviruses.Conclusions:Potential NAbs with similar key amino acids to those of gp41 fusion domain and gp120/gp41 interface might present in the HIV-1 infected patient with broad neutralizing antibodies.
