Effects of CVA16 infection on m 6A methylation-related protein expression and localization
10.3760/cma.j.cn112309-20200214-00062
- VernacularTitle:CVA16感染对m 6A甲基化相关蛋白表达和定位的影响
- Author:
Weiyu LI
1
;
Xi JIANG
;
Xuelin ZHENG
;
Wenbing ZHU
;
Zhuohang LIU
;
Hui LI
;
Xuemei ZHANG
;
Zhongxiang WU
;
Jie SONG
;
Shaozhong DONG
Author Information
1. 中国医学科学院 北京协和医学院，医学生物学研究所，云南省重大传染病疫苗研发重点实验室，昆明 650118
- From:
Chinese Journal of Microbiology and Immunology
2020;40(7):530-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether coxsackievirus A 16 (CVA16) infection would affect the expression of N6-methyladenosine (m 6A) methylation-related proteins in human bronchial epithelial cells (16HBE), ICR suckling mice and SCRBA2 humanized mice and influence their subcellular localization. Methods:CVA16 was used to infect 16HBE cells at a multiplicity of infection (MOI) of 0.1 and mice at 10 7 CCID 50/ml. Changes in the expression of methyltransferases, demethylases and methylated reading proteins were analyzed by Western blot. Cellular localization of these proteins was observed using immunofluorescence. Results:The expression of m 6A methylation-related proteins was gradually reduced in CVA16-infected cells with time, but showed no obvious change in ICR suckling mice or SCRBA2 humanized mice. After infection, m 6A methylation-related proteins were redistributed in both the nucleus and cytoplasm and even degraded. Conclusions:CVA16 replication in host cells altered the expression and cellular localization of m 6A methylation-related proteins, which indicated that m 6A modification might be a new potential target for enterovirus therapy.
