Detection of IgG antibody affinity in suspected cases of measles and rubella in Beijing
10.3760/cma.j.cn112309-20200329-00160
- VernacularTitle:北京市麻疹、风疹疑似病例IgG抗体亲和力水平检测
- Author:
Xiang GAO
1
;
Jie GAO
;
Ping ZHANG
;
Jinglin ZHOU
;
Lin ZOU
;
Chong ZHANG
;
Ling TONG
;
Guofeng ZHANG
;
Yanchun WANG
;
Jianguo WANG
;
Meng CHEN
Author Information
1. 北京市通州区疾病预防控制中心中心实验室 101100
- From:
Chinese Journal of Microbiology and Immunology
2020;40(6):465-469
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the causes of immune failure in the population with high vaccination rate of measles and rubella vaccine in Beijing by detecting the IgG antibody affinity in suspected cases of measles and rubella.Methods:Serum samples of 276 suspected cases of measles and rubella were tested for IgM and IgG antibodies by enzyme-linked immunosorbent assay (ELISA). The affinity of IgG antibody was detected, and the relative affinity index was calculated.Results:Among the 276 suspected cases, 104 were measles and 108 were rubella. Six measles cases had vaccination history and were caused by primary immunization failure ( n=3) and secondary immunization failure ( n=3). Twelve rubella cases had vaccination history and were due to primary immunization failure ( n=4) and secondary immunization failure ( n=8). Specific high-affinity antibodies were detected in nine measles cases and seven rubella cases without vaccination history, which indicated that these cases were reinfected. In the cases without measles or rubella, other pathogenic infections including mixed infections were detected, which were mainly caused by EB virus. Conclusions:Both primary and secondary immunization failure occurred in the population with immunization history. Reinfection was found in the patients who had not received vaccination against measles or rubella. Other pathogenic infections were existed among the cases without measles or rubella. Thus, misdiagnosis was responsible for the increased proportion of measles and rubella patients with immunization history in suspected cases in recent years. Full-course vaccination was conducive to produce high-affinity antibodies against measles and rubella. A supplementary vaccination campaign should be launched to consolidate the immune barrier against measles and rubella in key population or high-risk population, aiming to block the circulation of measles virus and achieve the goal of eliminating measles.
