Laboratory confirmation of an outbreak of respiratory infectious disease caused by human adenovirus 3 in Beijing
10.3760/cma.j.cn112309-20191125-00396
- VernacularTitle:北京地区一起3型腺病毒引起发热暴发疫情的实验室确诊
- Author:
Lin ZOU
1
;
Xiang GAO
;
Jianguo WANG
;
Chong ZHANG
;
Ling TONG
;
Lili YANG
;
Yanna YANG
;
Yanchun WANG
;
Hongjun LI
;
Tiegang ZHANG
;
Guofeng ZHANG
Author Information
1. 北京市通州区疾病预防控制中心 101100
- From:
Chinese Journal of Microbiology and Immunology
2020;40(5):355-358
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To confirm the possible pathogen causing an outbreak of respiratory infectious disease in Beijing.Methods:Oropharyngeal swabs were collected from 14 cases with fever and detected by RT-PCR for respiratory viruses and bacteria. For specimens positive for adenoviruses, Fiber, Hexon and Penton gene fragments were amplified with specific primers and sequenced. BLAST and phylogenetic tree were used for sequence analysis.Results:All of the 14 specimens were adenovirus-positive. BLAST analysis of the sequences of Fiber, hexon and Penton genes showed that the 14 cases were all caused by adenovirus 3. The phylogenic tree analysis indicated that this adenovirus was closely related to an adenovirus of 3a51 genotype (GenBank No: KF268123) isolated in the USA in 2007.Conclusions:Human adenovirus genotype 3a51 caused this outbreak of respiratory infectious disease in Beijing.
