Identification and characterization of Escherichia coli strains producing a novel Shiga toxin 2k subtype in goat feces in Lanling county, Shandong province
10.3760/cma.j.cn112309-20200323-00135
- VernacularTitle:山东省兰陵县羊粪中产志贺毒素2k新亚型大肠埃希菌的检出及其分子特征分析
- Author:
Deshui JIANG
1
;
Xi YANG
;
Hongbo JIAO
;
Bufang ZHANG
;
Yanyu PAN
;
Lijiao CAO
;
Yuhan ZHANG
;
Bin HU
;
Jun HU
;
Yanwen XIONG
Author Information
1. 山东省兰陵县疾病预防控制中心，临沂 277700
- From:
Chinese Journal of Microbiology and Immunology
2020;40(5):350-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the prevalence and molecular characteristics of Escherichia coli ( E. coli) producing a novel Shiga toxin 2k subtype in goat in Lanling county, Shandong province. Methods:In November 2019, 512 goat fecal samples were collected from different households in Lanling county, Shandong province. After enriched with EC broth, stx-positive samples were detected by PCR and inoculated in CHROMagar? ECC agar and CHROMagar? STEC agar. The whole genomes of stx-positive strains were sequenced. Based on the genomic senquences, the stx subtype, serotype, multi-locus sequence type and virulence genes of each strain were analyzed. Results:Eighty-six strains of Shiga toxin-producing E. coli (STEC) were isolated from 512 goat fecal samples. Five stx subtypes were identified and 37 strains were positive for stx2k. The 86 STEC strains belonged to 20 O∶H serotypes and 18 different sequence types (STs). Conclusions:STEC strains circulating in goats in Lanling county, Shandong province were heterogeneous in stx subtypes, serotypes and virulence gene profiles, and a certain proportion of strains producing a novel Shiga toxin 2k subtype were detected.
