Molecular mechanism of β-1, 4-galactosyltransferase 6 in regulating lymphocyte migration
10.3760/cma.j.cn112309-20191114-00376
- VernacularTitle:β-1，4-半乳糖基转移酶6在淋巴细胞迁移中的分子机制研究
- Author:
Yaya PIAN
1
;
Zhenxiang GAO
;
Jingjing NIE
;
Jihong HU
Author Information
1. 北京医院 国家老年医学中心 国家卫生健康委临床检验中心 中国医学科学院 老年医学研究院 100730
- From:
Chinese Journal of Microbiology and Immunology
2020;40(3):206-210
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the molecular mechanism of β-1, 4-galactosyltransferase 6 (β4galt6) in regulating lymphocyte migration under inflammatory conditions.Methods:CRISPR/Cas9 system was used to knock out the β4galt6 gene of mouse islet vascular endothelial cells (MS1). Adhesion assay was performed to compare the adhesion ability of lymphocytes to wild-type cells and gene knockout cells. Expression of adhesion molecules on the surface of wild-type cells and gene knockout cells were compared using RT-PCR and flow cytometry. Transwell model was used to compare the transmigration ability of lymphocytes across wild-type cells and gene knockout cells.Results:The β4galt6 gene knockout cell line, β4galt6 KO, was successfully constructed. The percentage of lymphocytes adhereing to wild-type MS1 cells was significantly higher than that to β4galt6 KO cells under inflammatory conditions. The expression of adhesion molecules including intercellular adhesion molecule 1 (ICAM1), vascular cell adhesion molecule 1 (VCAM1) and P-selectin on the surface of wild-type MS1 cells was significantly higher than that on β4galt6 KO cells. Moreover, the percentage of lymphocytes passing through wild-type MS1 cells was significantly higher than that through β4galt6 KO cells.Conclusion:Under inflammatory conditions, β4galt6 could promote the migration of lymphocytes.
