Effects of Mycobacterium tuberculosis liposaccharide ManLAM on CE protein-induced B cell activation
10.3760/cma.j.cn112309-20190305-00048
- VernacularTitle:结核分枝杆菌脂糖ManLAM对CE蛋白抗原诱导的B细胞活化的影响
- Author:
Wei LUO
1
;
Qian WANG
;
Yanqing DU
;
Chunhui YUAN
Author Information
1. 天津医科大学总医院检验科 300052
- From:
Chinese Journal of Microbiology and Immunology
2020;40(2):129-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence and potential mechanism of mannose-capped lipoarabinomannan (ManLAM) to B cells responding to Mycobacterium tuberculosis (Mtb) infection. Methods:B cells were separated from patients with active pulmonary tuberculosis using magnetic beads and then stimulated with ManLAM in combination with CE protein. Flow cytometry was performed to evaluate the apoptosis, proliferation and activation of B cells. The secretion of cytokines and CE protein-specific IgG subclasses were detected by ELISA. ELISPOT assay was used to analyze the influence on the differentiation of B cells into CE protein-specific antibody-secreting cells (ASCs).Results:ManLAM inhibited the CE protein-induced proliferation and activation of B cells and the production of pro-inflammatory cytokines, resulting in significantly increased secretion of the immunosuppressive cytokine IL-10. It also inhibited the differentiation of B cells into CE protein-specific IgG secretory cells, but had no significant influence on the differentiation to IgM secretory cells. Moreover, ManLAM inhibited the secretion of CE protein-specific IgG1 and IgG3 and induced the secretion of immunosuppressive IgG4 via TLR2.Conclusions:This study suggested that ManLAM could inhibit the anti-tuberculosis immune response of B cells, which provided new theoretical reference for better understanding the immune escape mechanism in Mtb infection.
