Identification of Bacterial and Fungal Isolates by Sequence Analysis of 16S rRNA and Internal Transcribed Spacer.
10.5145/KJCM.2010.13.1.34
- Author:
Younhee PARK
1
;
Hee Bong SHIN
;
Chang Ki KIM
;
Kyoung Ho ROH
;
Jong Hwa YUM
;
Dongeun YONG
;
Seok Hoon JEONG
;
Kyungwon LEE
Author Information
1. Department of Laboratory Medicine, Kwandong University College of Medicine, Goyang, Korea.
- Publication Type:Original Article
- Keywords:
16S rRNA;
Internal transcribed spacer;
Nucleotide sequence;
Bacterial identification
- MeSH:
Bacteria;
Base Sequence;
Databases, Nucleic Acid;
Fungi;
Microbial Sensitivity Tests;
Molecular Biology;
Sequence Analysis
- From:Korean Journal of Clinical Microbiology
2010;13(1):34-39
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Accurate and rapid identification of pathogens is one of the most important tasks of the clinical microbiology laboratory, and, in cases of rare pathogens, the identification is difficult and time-consuming upon the use of conventional methods alone. Herein, we will report our molecular work involving the identification of bacteria and fungi. METHODS: Sixty bacterial isolates had been collected from November 2004 to May 2007, and 15 fungal isolates had been collected from September 2005 to May 2007. Species identifications were performed using sequence analyses of the 16S rRNA region of bacteria and the internal transcribed spacer (ITS) region of fungi. The data were compared with those of GenBank (http://www.ncbi.nlm.nih.gov/) or EMBL (http://www.ebi.ac.uk/embl/). RESULTS: Sixty bacterial isolates included: 23 isolates with genus information (group 1), 17 isolates (group 2) that were too fastidious for genus or species identification, 16 isolates (group 3) with results from identification kits having low confidence, and 4 isolates (group 4) with odd antibiograms according to the species. In 58 of 60 isolates, identification of the genus or species could be obtained using molecular genetic methods. Thirty-eight isolates (63%) and 20 (33%) of 58 isolates could be identified at the species and genus levels, repectively. Among the total of 15 fungal isolates, 11 (73%) and 4 (27%) isolates were identified at the species and genus levels, respectively. CONCLUSION: 16S rRNA and ITS sequencing analyses are very useful for identifying the species or genus of a pathogenic microorganism in the clinical microbiology laboratory.
