Application of high-throughput second-generation gene sequencing in the diagnosis of pulmonary infection after organ transplantation
10.3760/cma.j.cn421203-20200331-00099
- VernacularTitle:高通量二代基因测序技术在器官移植术后肺部感染病原诊断的应用观察
- Author:
Hanlan LU
1
;
Mingxing SUI
;
Wenyu ZHAO
;
Rui CHEN
;
Jiazhao FU
;
Li ZENG
;
Youhua ZHU
;
Lei ZHANG
Author Information
1. 海军军医大学附属长海医院器官移植中心，上海 200433
- From:
Chinese Journal of Organ Transplantation
2020;41(7):388-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the application of high-throughput second-generation gene sequencing technology based upon metagenomics in the diagnosis of pulmonary infection after organ transplantation.Methods:From June 2016 to January 2020, clinical records were retrospectively reviewed for 34 renal and liver transplant recipients hospitalized for pulmonary infection. From June 2016 to December 2018, they were assigned as group A (n=20) of traditional pathogen detections. From January 2019 to January 2020, 14 cases in group B were sequenced by high-throughput second-generation technology. The detection rate, sensitivity and specificity, the return time of detection results, the average length of stay and the mortality of 28 days between two groups were analyzed.Results:No significant inter-group difference existed in clinical data (age, gender, antibody induction method, immunosuppressant use, etc.). As compared with group A, the positive detection rate of etiology and the the sensitivity were higher in group B and the differences in specificity were statistically insignificant. The return time of test results in group B was significantly shorter than that in group A. And the difference was statistically significant. The average hospitalization stay and 28-day mortality of group B were lower than those of group A. And the differences were statistically significant.Conclusions:High-throughput second-generation gene sequencing technology can improve the detection rate of pulmonary infection after organ transplantation. Providing a " precise and accurate" direction for disease treatment, it is a useful supplement to traditional diagnostic methods.
