Clinical values of 16S rRNA and ITS gene sequencing for pathogenic identification of renal transplant recipients with urinary tract infection
10.3760/cma.j.cn421203-20200319-00078
- VernacularTitle:16S rRNA及ITS基因测序在肾移植受者泌尿系感染病原微生物检测中的意义
- Author:
Naiqian CUI
1
;
Yi ZHOU
;
Wenfeng DENG
;
Hengrui ZHAO
;
Yuan ZHANG
;
Renfei XIA
;
Rumin LIU
;
Jian XU
;
Yun MIAO
Author Information
1. 南方医科大学南方医院 南方医科大学第一临床医院，广州 510515
- From:
Chinese Journal of Organ Transplantation
2020;41(7):393-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Objective To explore the clinical values of next-generation sequencing (NGS) in bacterial 16S rRNA region and fungal ITS region for diagnosing and treating urinary tract infection (UTI) in renal transplant recipients.Methods:A total of 90 mid-stream clean-catch urine samples were collected from renal transplant recipients who were diagnosed with UTI at Hospital from January 2017 to December 2019. Each sample was equally divided and tested via NGS method and traditional urine culture separately. The results of pathogen test and detection rate were analyzed and compared.Results:And 21/90 sample were considered to be contaminated due to the identification of three or more kinds of microorganisms by culture. And among the remaining 69 samples, 36 (52.17%) cases tested positive by 16S rRNA sequencing, 25 (36.23%) positive by urine bacterial culture; meanwhile, 34(49.28%) tested positive by ITS sequencing and 4(5.80%) positive by urine fungal culture.Conclusions:The detection rate of both bacteria and fungi in NGS microorganism testing is higher than that in traditional urine culture ( P< 0.05). For renal transplant recipients with UTI, NGS microorganism testing is an effective supplement for traditional urine culture. Improving the detection rate and accuracy of etiology may enable an optimization of individualized treatment.
