An experiment on the effect of endostar microbubble combined with focused ultrasound radiation on colon canear liver metastases
10.3760/cma.j.cn113855-20191118-00669
- VernacularTitle:载血管生成抑制剂脂质微泡联合聚焦超声定向辐照治疗结肠癌肝转移的实验研究
- Author:
Houdong WANG
1
;
Guangen YANG
;
Xiufeng ZHANG
;
Jianming QIU
;
Zhenfeng LU
;
Yanyan YU
;
Zhong SHEN
Author Information
1. 杭州市第三人民医院肛肠科 310009
- From:
Chinese Journal of General Surgery
2020;35(8):644-648
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the anti-tumor efficacy of endostar microbubble combined with focused ultrasound radiation in colon cancer liver metastases.Method:29 mice with colon cancer liver metastasis were randomly divided into four groups. Group 1(8 mice), as the control group. Group 2(7 mice) were treated only with ultrasonic radiation. Group 3 (7 mice) treated with the ultrasonic radiation combined with SonoVue microbubbles without carrying any medicine. Group 4(7 mice), treated with the ultrasonic radiation combined with microbubbles carrying endostar. The mice were sacrificed and the tumor specimens were weighted on the 12 days after ultrasound radiation. Immunohistochemistry was used to assess CD34 expression within the metastatic tumor.Results:The tumor weight in group 4 (0.79±0.49)g was significantly lower than that in group 1 (2.67±0.61)g, group 2 (2.60±0.60)g and group 3 (1.74±0.33)g ( F=20.629, P<0.01). The liver metastatic tumor weight in group 4(0.55±0.16) g was much lower than that in group 1 (1.47±0.22)g, group 2(1.42±0.28) g and group 3 (0.95±0.27)g ( F=23.758, P<0.01). There was no obvious difference among the four groups in the number of nodules of metastatic tumor in liver ( F=0.167, P=0.918). The level of CD34 in group 4 were (8 037±1 708) , significantly lower than that in any other group, ( F=15.779, P<0.01). Conclusion:Endostar microbubble combined with focused ultrasound radiation decreases tumor angiogenesis in liver metastasis, and inhibits the growth of both primary and metastatic tumor.
