The diagnostic value of flow cytometry in patients with lymphoma associated hemophagocytic syndrome
10.3760/cma.j.cn112138-20200605-00560
- VernacularTitle:流式细胞分析对以噬血细胞综合征为首发症状的非霍奇金淋巴瘤的诊断价值分析
- Author:
Wenli ZHANG
1
;
Lei XU
;
Qian ZHANG
;
Fengluan ZHONG
;
Jing DAI
;
Jia FENG
;
Lihua SUN
;
Haichan XU
;
Wenyue XIE
;
Hongyu ZHANG
Author Information
1. 北京大学深圳医院血液内科 518000
- From:
Chinese Journal of Internal Medicine
2020;59(12):976-981
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the reliability of flow cytometry (FCM) for diagnosing lymphoma associated hemophagocytic syndrome (LAHS).Method:The clinical data in 57 patients with hemophagocytic lymphohistiocytosis (HLH)were retrospective analyzed at Peking University Shenzhen Hospital from July 2010 to July 2019. All patients were performed bone marrow FCM and bone marrow pathological examination before final diagnoses were made. The golden diagnosis criterion was based on clinical, biochemical and histopathological evidence, which was regarded as the standard to evaluate the sensitivity and specificity of FCM analysis in diagnosing LAHS.Results:Among 57 cases, 36 cases were eventually diagnosed with LAHS, including 15 B-cell lymphoma(14 diffuse large B-cell lymphoma, 1 B-cell lymphoma with reactive T-cell hyperplasia), 13 aggressive NK/T cell lymphoma/leukemia, 2 cases of gamma-delta T-cell lymphoma, 4 angioimmunoblastic T-cell lymphoma, 1 enteropathy-associated peripheral T-cell lymphoma and 1 anaplastic T-cell lymphoma. Lymphoma cells in bone marrow were detected in all patients by FCM except one ENTCL patient. The sensitivity and the specificity of FCM in LASH compared to bone marrow biopsy were 97.2%( P=0.014)and 90.5%( P=0.488) respectively. In the other 21 non-LAHS patients, T cell receptor Vβ (TCRVβ) rearrangement was detected in 2 patients with Epstein-Barr virus (EBV) associated primary HLH. Conclusions:FCM effectively detects lymphoma cells in bone marrow of lymphoma patients with LHL, suggesting that FCM could be an important indicator for the diagnosis of LAHS. FCM also has the advantage in differentiating LAHS from other HLH.
