Effect of dexmedetomidine on excitability of fast-spiking interneurons in primary somatosensory cortex of mouse brain
10.3760/cma.j.cn131073.20191123.00615
- VernacularTitle:右美托咪定对小鼠大脑初级体感皮层快放电抑制性中间神经元兴奋性的影响
- Author:
Jiali TANG
1
;
Bo TANG
;
Yuguang HUANG
Author Information
1. 中国医学科学院 北京协和医院麻醉科 100730
- From:
Chinese Journal of Anesthesiology
2020;40(6):698-702
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of dexmedetomidine on the excitability of fast-spiking interneurons in the primary somatosensory cortex of mouse brain.Methods:Eleven healthy C57BL/6 mice of either sex, at postnatal days 15-21, were used.The acute brain slices of primary somatosensory cortex were prepared and incubated in artificial cerebrospinal fluid.The whole-cell patch-clamp was established on fast-spiking interneurons and excitatory neurons in primary somatosensory cortex layer 4 separately.The membrane potential and action potential threshold from fast-spiking interneurons and spontaneous inhibitory postsynaptic currents from excitatory neurons were recorded before application of dexmedetomidine (10 μmol/L)and at 5 min after application of dexmedetomidine.Results:Compared with that before application of dexmedetomidine, no significant change was found in the membrane potential and action potential threshold of fast-spiking interneurons and frequency and amplitude of spontaneous inhibitory postsynaptic currents at 5 min after application of dexmedetomidine( P>0.05). Conclusion:The analgesic mechanism of dexmedetomidine may not be related to the excitability of fast-spiking interneurons in the primary somatosensory cortex of the mouse brain.
