Effects of exogenous biliverdin on expression of Litaf in PC12 cells subjected to oxygen-glucose deprivation and restoration
10.3760/cma.j.cn131073.20190815.00323
- VernacularTitle:外源性胆绿素对氧糖缺失-复氧复糖损伤PC12细胞Litaf表达的影响
- Author:
Junjie LI
1
;
Zhiyao ZOU
;
Lijia PENG
;
Jing LUO
;
Li XIONG
;
Jia LIU
;
Tinghua WANG
;
Jianlin SHAO
Author Information
1. 昆明医科大学第一附属医院麻醉科 650000
- From:
Chinese Journal of Anesthesiology
2020;40(3):347-349
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of exogenous biliverdin (BV) on the expression of Litaf in PC12 cells subjected to oxygen-glucose deprivation and restoration (OGD/R).Methods:PC12 cells were seeded in a 96-well cell culture plate at a density of 1×10 4 cells/well for 3 days and were divided into 3 groups ( n=18 each) by a random number table method: control group (group C), OGD/R group, and biliverdin group (BV group). Group C was incubated in a 37 ℃ incubator (95% air+ 5%CO 2) for 6 h. To establish the OGD/R model, cells were incubated with sugar-free medium in a 37 ℃ incubator (95% air+ 5%CO 2) for 2 h, and the medium was then replaced with normal medium and cells were continuously incubated in a 37 ℃ incubator (95% N 2+ 5% CO 2). In BV group, 2 μg/ml biliverdin was added immediately after oxygen-glucose restoration.Cells in 6 wells in each group were selected at 6 h of restoration for determination of the expression of Litaf protein and mRNA (by real-time polymerase chain reaction) and tumor necrosis factor-alpha (TNF-α) concentration (by enzyme-linked immunosorbent assay). Results:Compared with group C, the expression of Litaf protein and mRNA was significantly up-regulated, and TNF-α concentration in supernatant was increased in group OGD/R ( P<0.05). Compared with group OGD/R, the expression of Litaf protein and mRNA was significantly down-regulated, and TNF-α concentration in supernatant was decreased in group BV ( P<0.05). Conclusion:The mechanism by which exogenous biliverdin reduces OGD/R damage to PC12 cells is related to inhibiting up-regulated expression of Litaf and alleviating the inflammatory responses.
