Effects of CD34 + cell transplantation on radiation-induced brain injury and relationship with activity of astrocytes in rats
10.3760/cma.j.issn.0254-1416.2020.02.014
- VernacularTitle:CD34 ＋细胞移植对大鼠放射性脑损伤的影响及其与星形胶质细胞活性的关系
- Author:
Yi SUN
1
;
Haiyun WANG
;
Mingshu ZHAO
;
Xuelong JIN
;
Wei HUA
Author Information
1. 天津医科大学三中心临床学院 天津市第三中心医院麻醉科 300170
- From:
Chinese Journal of Anesthesiology
2020;40(2):182-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of CD34 + cell transplantation on radiation-induced brain injury (RIBI) and the relationship with the activity of astrocytes in rats. Methods:Healthy adult male Sprague-Dawley rats, weighing 210-230 g, were divided into 3 groups ( n=36 each) using a random number table method: control group (C group), RIBI group, and CD34 + group.RIBI model was established by computed tomography (CT) scanning in anesthetized rats.Another 6 rats were selected, and CD34 + cells were eluted by flow cytometry and labeled with BrdU.CD34 + cells were transplanted at day 7 after establishing the model.Brain tissues were obtained at 7, 14 and 28 days after establishing the model in C and RIBI groups and at 14 and 28 days after establishing the model in CD34 + group for determination of Evans blue (EB) extravasation ratio and expression of GFAP (by immuno-histochemistry). Results:Compared with group C, the EB extravasation ratio was significantly increased after establishing the model, and the expression of GFAP was up-regulated in group RIBI ( P<0.05), and no significant change was found in EB extravasation ratio after establishing the model in group CD34 + ( P>0.05). Compared with group RIBI, the EB extravasation ratio was significantly decreased after establishing the model, and the expression of GFAP was down-regulated in group CD34 + ( P<0.05). Conclusion:CD34 + cell transplantation can reduce RIBI, and the mechanism may be related to inhibiting the activity of astrocytes in rats.
