Effects of subanesthetic concentration of sevoflurane on plasticity of dendritic spines in prefrontal cortex neurons of juvenile rats
10.3760/cma.j.issn.0254-1416.2020.01.019
- VernacularTitle:亚麻醉浓度七氟烷对青少年期大鼠前额皮质神经元树突棘可塑性的影响
- Author:
Junming LU
1
;
Yanxin CHEN
;
Tianyun ZHAO
;
Xingrong SONG
;
Wei WEI
;
Chuanxiang LI
Author Information
1. 南方医科大学第三附属医院麻醉科，广州 510515
- From:
Chinese Journal of Anesthesiology
2020;40(1):78-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of subanesthetic concentration of sevoflurane on the plasticity of dendritic spines in the prefrontal cortex neurons of juvenile rats.Methods:Thirty-six clean-grade male Sprague-Dawley rats, aged 24 days, weighing 50-60 g, were divided into control group (group C) and sevoflurane anesthesia group (group S) using a random number table method, with 18 rats in each group.Group S inhaled 1.2% sevoflurane and 50% oxygen (flow rate 1 L/min) for 3 h, while group C inhaled 50% oxygen (flow rate 1 L/min) for 3 h. Open-field test and Morris water maze test were performed at 3 days after anesthesia.Animals were sacrificed, and brain samples were then taken for determination of the number of apoptotic neurons in layer Ⅱ-Ⅲ of the prefrontal cortex, density of dendritic spines, and expression of postsynaptic density protein 95 and gephyrin by TUNEL staining, Golgi staining or Western blot.Results:Compared with group C, no significant change was found in total distance or time of staying at the central region in the open-field test or the average swimming velocity, escape latency or the number of apoptotic neurons in the Morris water maze test ( P>0.05), and the density of dendritic spines was significantly increased, and the expression of postsynaptic density protein 95 and gephyrin was up-regulated in group S ( P<0.05). Conclusion:Subanesthetic concentration of sevoflurane can enhance the plasticity of dendritic spines in the prefrontal cortex neurons of juvenile rats.
