Study on aging-associated changes of adiponectin in different organs and tissues of mice
10.3760/cma.j.issn.0254-9026.2020.05.022
- VernacularTitle:脂联素在小鼠各脏器组织中增龄性变化的研究
- Author:
Guoqing FAN
1
;
Qiugeng OUYANG
;
Lyutao ZENG
;
Ju CUI
;
Huan XI
;
Jianping CAI
Author Information
1. 北京医院 国家老年医学中心 国家卫生健康委北京老年医学研究所 中国医学科学院老年医学研究院 100730
- From:
Chinese Journal of Geriatrics
2020;39(5):582-586
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the trends of changes in oxidative stress and inflammatory factor levels in intestine tissues of mice during aging, and to explore their significance in the aging process.Methods:Four-month-old and 20-month-old male C57BL6 mice were used, with 3 mice in each group.The mice were sacrificed under anesthesia, and serum, liver, spleen, kidney, intestine and lung tissues were collected.Age-related changes in expression levels of inflammation-related proteins in serum, intestine, liver, spleen, kidney and lung were detected and analyzed by cytokine antibody arrays.Immunohistochemistry was used to examine aging-related changes of adiponectin in tissues and 8-oxoguanine (8-oxoG) levels in intestinal tissues.Results:Cytokine microarrays revealed that levels of adiponectin in the intestine and serum were significantly higher in 20-month-old mice than those in 4-month-old mice ( t=5.370, 23.490, both P<0.05). Immunohistochemistry results showed that expression levels of 8-oxoG in 20-month-old mice were significantly higher than those in 4-month-old mice ( t=8.785, P<0.05). Furthermore, in intestine tissue, expression levels of adiponectin were significantly higher in 20-month-old mice, compared with 4-month-old mice ( t=24.530, P<0.05). However, there was no significant difference in adiponectin levels in spleen, kidney, lung and liver tissues between young and aged mice. Conclusions:Adiponectin levels in serum and intestine tissue increase with age and have the potential to be used as a specific intestinal tissue biomarker of aging.
