In vitro targeted transfection of recombinant adenovirus vectors containing the hypoxia inducible factor-1α gene in rat hypoxic brain microvascular endothelial cells
10.3760/cma.j.issn.0254-9026.2020.05.023
- VernacularTitle:重组腺病毒缺氧诱导因子-1α体外靶向性转染大鼠缺氧脑微血管内皮细胞的研究
- Author:
Minglu JIN
1
;
Tao TAO
;
Zhehua ZOU
;
Jian XU
;
Liling GU
;
Yu FU
;
Jian MEI
Author Information
1. 贵州省人民医院康复医学科(现在重庆医科大学附属第一医院綦江医院神经内科，綦江 401420)，贵阳 550002
- From:
Chinese Journal of Geriatrics
2020;39(5):587-590
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the transfection of recombinant adenovirus vectors containing the hypoxia inducible factor-1α gene (AdHIF-1α)in rat brain microvascular endothelial cells(BMECs) and to provide a theoretical basis for the treatment of hypoxic BMECs by AdHIF-1α.Methods:Rat BMECs were isolated, identified, and cultured in a maintenance medium containing 100 μmol/L cobalt dichloride (CoCl 2), establishing a hypoxia model of BMECs; then AdHIF-1α was transfected into hypoxic BMECs.The transfection of fluorescent protein was observed under a fluorescence microscope. Results:Transfection of AdHIF-1α into BMECs was monitored under a fluorescence microscope at 12 h, 24 h, 48 h and 72 h, respectively.Minor fluorescence began to appear at 12 h (0.13±0.01), and the fluorescence expression increased at 24 h (0.46±0.03, q=25.88, P<0.01), was most obvious at 48 h (0.97±0.05, q=40.00, P<0.01), and decreased at 72 h (0.38±0.02, q=46.28, P<0.01). Conclusions:Recombinant adenovirus vectors containing AdHIF-1α can be transfected into hypoxic BMECs in vitro.
