Relationship between YES-related protein 1 and prostate-specific antigen in castration-resistant prostate cancer
10.3760/cma.j.issn.0254-9026.2020.02.016
- VernacularTitle:去势抵抗前列腺癌患者中YES相关蛋白1与前列腺特异性抗原的关系
- Author:
Miaomiao WANG
1
;
Peikang WU
;
Yihao LIAO
;
Mingyang DU
;
Yuanjie NIU
;
Ning JIANG
Author Information
1. 天津医科大学第二医院泌尿外科 天津市泌尿外科研究所，天津 300211
- From:
Chinese Journal of Geriatrics
2020;39(2):192-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between YES-related protein 1(YAP1)and prostate-specific antigen(PSA)in human castration-resistant prostate cancer(CRPC), and explore the regulation mechanism of YAP1 on PSA.Methods:The luciferase reporter gene was used to detect the activity change of the PSA gene promoter region after the over expression of YAP1 in LNCaP and C4-2 cells.The effect of over expression of YAP1 gene on PSA protein in different prostate cancer cell lines was detected by Western blot(WB)method, and the effect of YAP1 silencing on PSA protein in C4-2 cells was observed.The Q-PCR method was used to further verify the expression change of PSA mRNA affected by YAP1 gene over expressed in C4-2 cells.Meanwhile, WB was used to explore the effect of YAP1 on androgen receptor(AR)in C4-2 cells.Results:After over expression YAP1 in CRPC, the luciferase experiment showed that the average C4-2 cell ratio of experimental group to control group was 3.17815892(>2 times, P<0.001). After Q-PCR detection of all over-expressed YAP1 gene fragments, the measured PSA mRNA values in the experimental groups were 2.306667, 1.553333333, 2.613333333, and 2.673333333, respectively, which were higher than those in the control group(1 time, P<0.001), indicating that the PSA expression was significantly increased.WB analysis showed that after C4-2 cells over expressed YAP1, the AR band was significantly enhanced in the experimental group compared with the control group, suggesting that the AR protein expression in the nucleus was significantly increased in the YAP1 over expression group. Conclusions:YAP1 might positively regulate the PSA expression in CRPC and have an ability to promote AR translocation into the nucleus.
