Rapid determination of various fat-soluble vitamins in serum by isotope dilution ultra high performance liquid chromatography-tandem mass spectrometry
10.3760/cma.j.cn321828-20190930-00216
- VernacularTitle:同位素稀释超高效液相色谱串联质谱法快速检测血清中多种脂溶性维生素含量
- Author:
Wanwan YI
1
;
Qiuyuan SHI
;
Congyan CHEN
;
Fang YUAN
;
Zhongwei LYU
;
Jin LIU
Author Information
1. 同济大学附属第十人民医院核医学科，上海 200072
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2020;40(10):599-602
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an analytical method for the simultaneous determination of five fat-soluble vitamins in serum using isotope dilution ultra high performance liquid chromatography-tandem mass spectrometry (ID-UPLC-MSMS).Methods:Fat-soluble vitamins were obtained from serum samples which collected from Shanghai Tenth People′s Hospital between April 2019 and August 2019 by the extraction method, and were detected by ID-UPLC-MSMS. The performance of the method was verified by referring to the relevant documents of the Clinical and Laboratory Standards Institute (CLSI).Results:The ID-UPLC-MSMS method for the rapid detection of various fat-soluble vitamins in serum was proposed and successfully verified. The linear range of the method: vitamin A: 25-2 500 μg/L, 25(OH)D 2: 2-200 μg/L, 25(OH)D 3: 2-200 μg/L, vitamin E: 0.25-50 mg/L, vitamin K1: 0.1-20 μg/L. The intra- and inter-assay precision standard deviations of the five analytes were within ± 15%, and the accuracy of the test results of the 25(OH)D 2 and 25(OH)D 3 standards was 96.44%-102.37%. Conclusion:The performance of ID-UPLC-MSMS method for the simultaneous determination of five fat-soluble vitamins is satisying, and the result is accurate and reliable, which suggested it can be used for the clinical sample.
