Study on the expression level of NLRP3 inflammasome in hepatic alveolar echinococcosis
10.3760/cma.j.cn113884-20191024-00347
- VernacularTitle:NLRP3炎性小体在肝泡型包虫病中表达水平的研究
- Author:
Xubiao PAN
1
;
Hongbin WANG
;
Zhixin WANG
;
Haining FAN
Author Information
1. 河南省中医院（河南中医药大学第二附属医院）普通外科二病区，郑州 450003
- From:
Chinese Journal of Hepatobiliary Surgery
2020;26(10):753-756
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression level and clinical significance of NLRP3 inflammasome associated protein and inflammatory factors in hepatic alveolar echinococcosis (HAE).Methods:A total of 21 HAE patients (case group) and 40 healthy subjects (health group) who underwent surgical treatment in the Affiliated Hospital of Qinghai University from January 2018 to August 2018 were selected and separated into case group and healthy group. In the case group, 12 were males and 9 were females, aged (35.20±7.50) years; in the healthy group, there were 24 males and 16 females, aged (35.19±14.48) years old. The expression of NLRP3, Caspase-1, IL-1β, IL-18 and IL-33 in normal liver tissues and the nodule tissues were detected by immunohistochemistry. The expression of IL-1β, IL-18 and IL-33 was detected in the serum of patients by ELISA. Statistical method was used to analyze the difference of each test index between the two groups.Results:The positivity and intensity of NLRP3, Caspase-1, IL-1β and IL-33 in marginal lesion tissue were more than normal liver tissue, while IL-18 was weaker. The differences were significant ( P<0.05). ELISA assay showed that the expression level of IL-33 in the case group was higher than that in the healthy group [177.88(168.87, 192.92) pg/ml vs 171.78(161.80, 181.93) pg/ml] with significant differences ( P<0.05). There were no significant differences in the levels of IL-1β and IL-18 in serum between case group and healthy group ( P>0.05). Conclusion:The abnormal expression of NLRP3 inflammasome associated protein and inflammatory factor in HAE suggested that the inflammatory body may be involved in the progression of the disease.
