Rabbit models for the study of local antibiotics irrigation on chronic proliferative cholangitis
10.3760/cma.j.cn113884-20191030-00355
- VernacularTitle:胆道局部抗生素冲洗治疗慢性增生性胆管炎的大白兔研究
- Author:
Yilei DENG
1
;
Wenjie MA
;
Fuyu LI
;
Menghao ZHOU
;
Longshuan ZHAO
Author Information
1. 郑州大学第一附属医院肝胆胰外科，郑州 450000
- From:
Chinese Journal of Hepatobiliary Surgery
2020;26(8):620-623
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of local antibiotics irrigation on chronic proliferative cholangitis (CPC).Methods:CPC model of rabbits was established. Rabbits were divided into CPC group (without local antibiotics irrigation, n=20) and experimental group (with local antibiotics irrigation, n=20). Only the gallbladder was removed, and 20 rabbits with free bile duct (sham operation) were used as normal control. The inflammatory conditions, proliferation of cholangiocytes, biliary fibrosis and biliary stones formation ability were analyzed. Results:Compared with CPC group, the relative expression of inflammation index lipopolysaccharide and interleukin-6 [(1.21±0.13) vs. (3.24±0.21), (1.52±0.22) vs. (3.10±0.23)], biliary cell proliferation index cyclooxygenase-2 and vascular endothelial growth factor [(2.15±0.12) vs. (4.07±0.22), (2.44±0.14) vs. (3.22±0.21)], fibrosis index transforming growth factor-β and Collagen-I [(2.44±0.28) vs. (4.36±0.44), (1.54±0.13) vs. (2.22±0.18)] and biliary stones formation index β-glucuronidase and Mucin 5AC [(1.74±0.20) vs. (3.42±0.31), (1.47±0.15) vs. (2.81±0.22)] were significantly decreased in experimental group (all P<0.05). Conclusion:Local antibiotics irrigation could inhibit CPC by inhibiting the chronic inflammation of the biliary tract and excessive proliferation of cholangiocytes and biliary fibrosis, and reducing the probability of biliary stone formation.
