Bone mesenchymal stem cells transplantation in the treatment of chronic pancreatitis of rats model
10.3760/cma.j.cn113884-20191229-00425
- VernacularTitle:骨髓间充质干细胞移植治疗大鼠慢性胰腺炎
- Author:
Yongyuan LI
1
;
Zhao LI
;
Yue DU
;
Xin WANG
Author Information
1. 天津市第五中心医院普通外科 300450
- From:
Chinese Journal of Hepatobiliary Surgery
2020;26(6):455-458
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the therapeutic effects and mechanism of bone mesenchymal stem cells(BMSCs)transplantation in the pancreas injury of chronic pancreatitis of rats.Methods:Based on the transplantation methods, all the rats were divided into 5 groups with 10 rats in each: control group, model group, submesopancreas treatment group, portal vein treatment group, tail vein treatment group. The pancreatic fibrosis and pathological score were evaluated. The percentage of PKH26, Pax4, Ngn3 positive-BMSCs in the pancreas, the percentages of double-labeled cells (PKH26 + /Pax4 + , PKH26 + /Ngn3 +) and the expression of α-SMA, collagen type I and III, IL-10 in pancreas were detected. Results:The pancreatic fibrosis and pathological score in treatment group were significantly lower than that of model group (both P<0.05). There was no significant difference of pancreatic fibrosis and pathological score in the other 3-treatment group ( P>0.05). The expression of PKH26, Pax4 and Ngn3 in tissues were much higher in the treatment group compared with model group and control group with significant differences (all P<0.05). There was no significant difference for Pax4 and Ngn3 in pancreas among the other 3-treatment group ( P>0.05). There were double-labeled cells (PKH26 + /Pax4 + , PKH26 + /Ngn3 +) with no significant differences among 3 treatment group ( P>0.05). The expression of α-SMA, collagen type I and III in tissues was lower in treatment group compared with model group with significant difference (all P<0.05), while the level of IL-10 in pancreatic tissue was higher in treatment group compared with model group with significant difference ( P<0.05). There was no significant difference for the expression of α-SMA, collagen type I, collagen type III and IL-10 in tissues among the 3 treatment group (all P>0.05). Conclusions:BMSCs have the therapeutic effects in the treatment of chronic pancreatitis. For the mechanism, the recruitments and engraftment of BMSC contributed to the injury of pancreatic tissue, BMSCs’ promotion to the pancreatic differentiation, and the inhibition of CP inflammation by autocrine or paracrine function.
