Effects and mechanism of estrogen receptor beta agonist on hyperdynamic circulation in cirrhotic rats with portal hypertension
10.3760/cma.j.cn113884-20190818-00266
- VernacularTitle:雌激素受体β激动剂对肝硬化门静脉高压大鼠高动力循环的调节作用及其机制
- Author:
Chenggang ZHANG
1
;
Bin ZHANG
;
Lin YAN
;
Chao LIANG
;
Cheng CHEN
;
Zhiyong WU
Author Information
1. 上海中医药大学附属岳阳中西医结合医院肝胆外科 200437
- From:
Chinese Journal of Hepatobiliary Surgery
2020;26(4):295-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of estrogen receptor beta agonist diarylpropionitrile (DPN) on mesenteric artery (MA) responsiveness and on Rho-kinase signaling pathway in cirrhotic portal hypertension rats, and to elucidate the mechanism of ER beta agonist in visceral hyperdynamic circulation in cirrhotic portal hypertension rats.Methods:Female rats were treated with bilateral ovariectomy, and CCl 4 was injected to establish cirrhotic portal hypertension model. After intervention, hemodynamic parameters and vascular reactivity of MA in each group were measured, and the levelof ER beta, Rho-kinase signaling pathway-related proteins and receptor desensitization-related proteins in MA in each group were detected. Results:DPN could significantly improve the hemodynamic parameters of ovariectomized cirrhosis rats and the response of MA to norepinephrine. DPN could increase the level of inhibited ROCK protein and decrease the expression of beta-arrestin-2 and the phosphorylation of ERK1/2.Conclusions:DPN could improve the responsiveness of MA to vasoconstrictor substances, and significantly improve the hyperdynamic circulation in ovariectomized cirrhosis rats. This effect may be related to DPN improving the desensitization of MA to vasoconstrictor receptors and the Rho-kinase pathway activation.
