Usefulness of Newly Developed Film Form Drug Delivery System that Inhibits Lens Epithelial Cell Proliferation.
- Author:
Hyun Woo KIM
1
;
Seok Jong LEE
;
Choun Ki JOO
Author Information
1. Laboratory of Ophthalmology and Visual Science, College of Medicine, The Catholic university of Korea, Seoul, Korea. ckjoo@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
After cataract;
Cataract;
Drug delivery system
- MeSH:
Cataract;
Chitosan;
Chromatography, Liquid;
Crystallins;
Drug Delivery Systems*;
Epithelial Cells*;
Fluorouracil;
Glycerol;
Humans;
Muramidase;
Polymers;
Water
- From:Journal of the Korean Ophthalmological Society
2003;44(5):1198-1204
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To develop a therapeutic drug delivery system which inhibits lens epithelial cells proliferation. METHODS: Chitosan, a natural polymer, which is biocompatible and biodegradable was mixed with glycerol with a certain ratio to make it an asceptic film form. Water content and degradability of this film was determined. During the construction of film, 5-fluorouracil (5FU) was added to make of film containing 5FU. Drug release was checked with high performance liquid chromatography (HPLC). Cell cytotoxicity of this film was determined with MTT (3-(4, 5-dimethylathiazol-2yl)-2, 5-diphenyl tetrasoliumbromide) assay. RESULTS: Newly constructed film did not show any cell cytotoxicity against human lens epithelial cells, HLE-B3. In the lysozyme-free PBS, 62% of C/Gly-1 and 53% of C/Gly-0.7 were degraded respectively after 4 weeks. CONCLUSIONS: This film was designed to be inserted between artificial crystalline and posterior capsule. With its transparency, this film may not interfere the vision of patient. This film is also completely degradable in the lysozyme containing condition. With these characteristics, this film may be used in the drug delivery system that inhibits the formation of after cataract.
