Screening of radiosensitive lipid metabolites in rat plasma
10.3760/cma.j.issn.0254-5098.2020.06.002
- VernacularTitle:大鼠血浆辐射敏感脂质代谢物的筛选
- Author:
Hua ZHAO
1
;
Xuelei TIAN
;
Cong XI
;
Ling GAO
;
Mei TIAN
;
Qingjie LIU
Author Information
1. 中国疾病预防控制中心辐射防护与核安全医学所 辐射防护与核应急中国疾病预防控制中心重点实验室，北京 100088
- From:
Chinese Journal of Radiological Medicine and Protection
2020;40(6):420-426
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen radiosensitive lipid metabolites in rat plasma and analyze their metabolic pathways in order to provide scientific basis for radiation damage biomarker.Methods:The whole body irradiation of 60Co γ rays was performed to rats with different doses of 0, 1, 3 and 5 Gy. The changes of lipids in plasma were detected by untargeted lipidomics method based on liquid chromatography coupled mass spectrometry. Results:Twenty plasma lipids were identified as the potential radiosensitive biomarkers at 7 days after irradiation, including 13 over-expressed lipids and 7 down-expressed lipids, where 12 lipids well responded to radiation doses.Conclusions:Lipid metabolites in rat plasma are significantly changed after exposure to γ rays, and the metabolic pathways of sphingolipid, glycerophospholipid and glycosylphosphatidylinositol (GPI) are significantly enriched.
