Study on the role of Aryl hydrocarbon receptor induced Th17 differentiation in dendritic cells in primary biliary cholangitis
10.3760/cma.j.cn141217-20200102-00003
- VernacularTitle:树突状细胞芳香烃受体诱导辅助性T细胞17分化在原发性胆汁胆管炎中的作用研究
- Author:
Chunhui SHE
1
;
Jing WANG
;
Bin LIU
Author Information
1. 青岛大学附属医院风湿免疫科 266000
- From:
Chinese Journal of Rheumatology
2020;24(7):433-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of Aryl hydrocarbon receptor (AhR) activated dendritic cell (DC) on T helper cell 17 (Th17) differentiation in primary biliary cholangitis (PBC) patients.Methods:Per-ipheral blood samples of 10 patients with PBC and 10 healthy people were collected. CD14 + mononuclear cells were isolated and induced to differentiate to DCs. After stimulated by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD), these DCs were co-cultured with the na?ve CD4 + T cells. Then, the proliferation activity of T cells was detected by Cell Counting Kit-8 (CCK-8) and cytokines related to Th17 differentiation were detected by quantitative real-time-polymerase chain reaction (qRT-PCR) and enzyme-linked immuno sorbent assay (ELISA). Results:A large number of DCs were obtained, and the microscopic observation showed that the cells were enlarged in size, irregular in shape, and numerous protrusions on the surface. The CD11 and CD14 double positive cells accounted for 96.48%. After co-culture, the proliferation capacity of na?ve CD4 + T cells in the PBC group was significantly higher than that in the healthy control group. Absorbance ( A) value measured by CCK-8 method at 72 hours (1.02±0.04) was significantly higher than that at 24 hours (0.69±0.04) and 48 hours (0.82±0.04), and the difference was statistically significant ( LSD- t1=10.2, P<0.05; LSD- t2=6.3, P<0.05). The expression level of na?ve CD4 + T cells IL-17 in the PBC patients group was(1.24±0.40) higher than that in the healthy control group (0.61±0.18), and the difference was statistically significant ( t=3.90, P<0.05). The expression level of IL-22 (0.96±0.46) was also higher than that of the healthy control group (0.42±0.43), and the difference was statistically significant ( t=2.76, P<0.05). The expression of IL-22 in the culture supernatant of the PBC patients group was (77.4±2.9) ng/ml, significantly higher than that of the healthy control group (49.6±4.0) ng/ml, the difference was statistically significant ( t=5.58, P<0.01). The expression level of IL-17 (170.9±2.7) ng/ml was also higher than that of the healthy control group (130.6±7.1) ng/ml, and the difference was statistically significant ( t=9.10, P<0.05). Conclusion:DCs activated by AhR can promote the differentiation of Th17 and the production of related cytokines. Thus, DCs activated by AhR may promote the occurrence of PBC.
