Potential mechanism of microRNA in hepatitis B virus infection by using genechip and public database data
10.3760/cma.j.cn311365-20200109-00009
- VernacularTitle:基于芯片和公共数据库数据分析微RNA在乙型肝炎病毒感染中的可能机制
- Author:
Shenglong LIN
1
;
Xiangmei WANG
;
Huaxi MA
;
Dongqing ZHANG
;
Wenjun WU
;
Jiahuang LIN
;
Ziyuan LIAO
;
Minghua LIN
;
Ruidan ZHENG
;
Haibing GAO
Author Information
1. 福建医科大学孟超肝胆医院肝内科，福州 350025
- From:
Chinese Journal of Infectious Diseases
2020;38(11):717-722
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the potential mechanism of microRNA (miRNA) in hepatitis B virus (HBV) infection.Methods:The peripheral blood samples were collected from four chronic hepatitis B (CHB) patients who visited Mengchao Hepatobiliary Hospital of Fujian Medical University in 2017, and those were also collected from four healthy controls. Affymetrix GeneChip microRNA 4.0 was applied to detect the expressions of miRNA between CHB patients and healthy controls. The CHB relative differential expressions of miRNA were obtained. The functions of CHB relative miRNA were analyzed by the combination of bioinformatics tools and public database data.Results:A total of seven miRNA were differentially expressed in the peripheral blood of CHB patients. Among them, miRNA-122-5p (log 2 fold change (log 2FC)=7.78, P=0.007 3), let-7c-5p (log 2FC=3.52, P=0.019 6), miRNA-6794-5p (log 2FC=1.15, P=0.033 2), and miRNA-1226-5p (log 2FC=0.68, P=0.034 3) were up-regulated, while miRNA-619-5p (log 2FC=-1.83, P=0.002 6), miRNA-1273g-3p (log 2FC=-2.69, P=0.025 1), and miRNA-4440 (log 2FC=-3.99, P=0.047 8) were down-regulated. Further analysis showed that these miRNA could directly interact with HBV gene sequence and impact the replication of the virus. Among them, miRNA-122-5p, miRNA-6794-5p and miRNA-1226-5p could negatively regulate target genes expression to influence the formation of ficolin-1 rich granule, ficolin-1 rich granule lumen, podosome and membrane ruffle, which participated in the cell membrane movement and cell-matrix adhesion. Conclusion:MiRNA could impact the molecular movement in the cell membrane and facilitate HBV entry to liver cells, playing an important supporting role in HBV infection process.
