Study on the serotype, drug sensitivity and virulence genes of clinical isolates of Salmonella in Zhejiang Province
10.3760/cma.j.cn311365-20191220-00420
- VernacularTitle:浙江省临床分离沙门菌血清型、药物敏感性和毒力基因的研究
- Author:
Yue WANG
1
;
Xiao CHEN
;
Ruonan WANG
;
Jun CHENG
;
Changgui SUN
;
Hongcui CAO
Author Information
1. 浙江大学医学院附属第一医院传染病诊治国家重点实验室，国家感染性疾病临床医学研究中心，杭州 310003
- From:
Chinese Journal of Infectious Diseases
2020;38(10):646-650
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the serotype distribution, drug resistance and the virulence gene profiles of clinical isolates of Salmonella in Zhejiang Province. Methods:A retrospective study of 463 clinical isolates of Salmonella in nine regions of Zhejiang Province from 2010 to 2017 was conducted. Their serotypes were detected using the Kauffmann-White scheme based on serological detection. Antibiotics susceptibility tests were carried out using K-B disk diffusion method. Eleven virulence genes were detected by polymerase chain reaction. Results:A total of 35 serotypes were identified among the 463 strains of Salmonella. The dominant serotypes were Salmonella typhimurium (41.90%(194/463)) and Salmonella enteritis (22.25%(103/463)). The resistance rate of Salmonella to ampicillin was the highest (66.52%(308/463)), followed by ampicillin/sulbactam (46.87%(217/463)), while low resistance rate to piperacillin/tazobactam was observed (3.24%(15/463)). All strains were sensitive to imipenem and meropenem. Finally, 188 strains (40.60%) of multi-drug resistance were found. The virulence genes hilA, ssrB, marT, siiD, sopB and pagN were found in all Salmonella strains. The virulence gene vexA was found only in Salmonella typhi and Salmonella Dublin; virulence gene icmF was mainly found in Salmonella enteritis. The virulence plasmid gene spvB and pefA were mainly found in Salmonella enteritidis and Salmonella typhimurium and invariably appeared in pairs. The virulence gene cdtB was mainly found in Salmonella typhi and Salmonella paratyphi A. Conclusions:Salmonella typhimurium and Salmonella enteritis are the main clinically isolated Salmonella strains in Zhejiang Province. The situation of multi-drug resistance is severe and a variety of virulence genes are highly detected.
