Anti-leukemia effect of icaritin and its mechanisms on cell line of children relapsed acute lymphoblastic leukemia
10.3760/cma.j.cn431274-20191229-01482
- VernacularTitle:淫羊藿素对儿童复发急性淋巴细胞白血病细胞的抗白血病效应及其机制
- Author:
Wenyong KUANG
1
;
Mincui ZHENG
;
Wanli LI
;
Haixia YANG
;
Ruijuan LI
Author Information
1. 湖南省儿童医院血液内科，长沙 410007
- From:
Journal of Chinese Physician
2020;22(6):866-870,874
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the anti-leukemia effect of icaritin on 697 cells of children relapsed acute lymphoblastic leukemia in vitro, and to analyze its possible mechanism. Methods:697 cells were cultured in vitro and divided into control group and icariin groups (5, 10, 20 μmol/L). After treated with icaritin, the cell proliferation was detected with cell counting kit-8 (CCK-8) method, and apoptosis was measured with Hoechst 33258 staining and Flow Cytometry Assay. Expression of Bcl-2, Cyt-C, Bax and Caspase3 proteins in 697 cell were detected by Western blot. Results:After treatment with various concentrations (5-20 μmol/L) of icaritin for different time-points (24, 48, 72 h), the growth inhibition rate exhibited a dose and time-dependent manner ( r=0.76, r=0.89); the apoptotic rate exhibited significant increase for 48 h ( P<0.05). Bcl-2 protein decreased significantly ( P<0.05), while Bax, Cyt-C and Caspase3 cleavage protein increased significantly in icaritin treated group for 48 h compared with control group ( P<0.05). Conclusions:Icaritin shows the effects of anti-leukemia by inducing apoptosis and inhibiting proliferation of 697 cell. Icaritin maybe induce apoptosis of 697 cell by down-regulation of Bcl-2 expression and up-regulation of the Bax, Cyt-C and cleaved Caspase 3 expression.
